Revvity, TRF4023C, LANCE Total eIF2α Detection Kit, 500 Assay Points

Overview
Please note control lysates are sold separately, catalog number TRF4008S.
Formats:

Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
Eukaryotic initiation factor 2 (eIF2) is a heterotrimer which is involved in the initiation of translation in eukaryotes. EIF2 binds to two other partners, GTP and Met-tRNAi, and forms the 43S pre-initiation complex upon binding the 40S subunit. Under conditions of cellular stress, the alpha subunit can be phosphorylated at S51 to downregulate protein translation. Application-Cell Signaling
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Molecular Modification-Total
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target Class-Phosphoproteins
Technology-TR-FRET
Therapeutic Area-Autoimmunity
Unit Size-500 Assay Points