Revvity, TRF4026M, LANCE Phospho-LCK (Tyr394) Detection Kit, 10,000 Assay Points

Overview
Please note control lysates are sold separately, catalog number TRF4026S.
Formats:

Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
LCK is a cytoplasmic phosphoprotein from the Src family of non-receptor tyrosine kinases. LCK regulates T cell activation by associating with CD4 and CD8 surface receptors on T cells. Phosphorylation at Y394 enhances kinase activity. LCK is associated with Immunodeficiency 22 and CD45 deficiency. No-death receptor apoptosis is also involves LCK. Application-Cell Signaling
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Molecular Modification-Phosphorylation
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-LCK
Target Class-Phosphoproteins
Technology-TR-FRET
Unit Size-10,000 Assay Points