Revvity, TRF4027M, LANCE Phospho-AMPKα (Thr172) Detection Kit, 10,000 Assay Points

Overview
Please note control lysates are sold separately, catalog number TRF4027S.
Formats:

Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
AMPKα is an isoform of AMP-activated protein kinase which plays a role in cellular energy homeostasis. Once bound by AMP or ADP, AMPK activations results in the stimulation of hepatic fatty acid oxidation, ketogenesis, and glucose uptake amongst other metabolic functions. Once the alpha subunit is phosphorylated at the T172 position, AMPK is considered activated. Application-Cell Signaling
Automation Compatible-Yes
Brand-LANCE Ultra
Detection Modality-LANCE
Molecular Modification-Phosphorylation
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-AMPKα
Target Class-Phosphoproteins
Technology-TR-FRET
Therapeutic Area-Cardiovascular Neuroscience
Unit Size-10,000 Assay Points