Revvity, TRF4028M, LANCE Phospho-STAT1 (Tyr701 ) Detection Kit, 10,000 Assay Points

Overview
Please note control lysates are sold separately, catalog number TRF4028S.
Formats:

Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
STAT1 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT1 is phosphorylated at Tyr-701 by receptor associated kinases, upon which STAT1 will dimerize (sometimes forming a heterodimer with STAT3), translocate to the nucleus, and work as transcription factors. Mutations of STAT1 result in either gain of function (resulting in CMC) or loss of function (causing impaired response to infection). Application-Cell Signaling
Automation Compatible-Yes
Brand-LANCE Ultra
Cellular or Signaling Pathway-JAK/STAT
Detection Modality-LANCE
Molecular Modification-Phosphorylation
Product Group-Kit
Sample Volume-15 µL
Shipping Conditions-Shipped in Blue Ice
Target-STAT1
Target Class-Phosphoproteins
Technology-TR-FRET
Therapeutic Area-Autoimmunity Immuno-oncology Oncology Respiratory
Unit Size-10,000 Assay Points