Sartorius, 110.5113-1.3, CIMac QA 0.1 mL Analytical Column (1.3 µm)

This high-performance analytical column is primarily intended for fast, efficient and reproducible analysis and in-process control of proteins, viral particles and other biomolecules under hydrophilic conditions throughout different purification steps.

CIMac Analytical Columns are intended for fast and reproducible HPLC monitoring and quantitation of large proteins, plasmid DNA, viruses, virus-like particles, and phages. They were primarily designed for the in-process and final control of various samples at different stages of the purification process. They can be operated at high volumetric flow rates (from 1 to 20 column volumes/min); therefore, the information about the product quantity and purity can be obtained within a few minutes. CIMac Analytical Columns are fast and reliable PAT tools, providing:
Rapid, high resolution analysis, achieved in minutes
Flow independent separation
High sensitivity and reproducibility
QA (quaternary amine) features:
Strong anion exchanger
Working range, pH 2–13
Binds molecules with predominantly negative charge, repels molecules with a predominantly positive charge
Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
High capacity for virus particles, IgM, exosomes
High efficiency removal of endotoxin and DNA removal
Outstanding for preparative applications or analytical finger-printing of process samples
Sanitizable with 1 M NaOH

Specifications
Applications:
Application: Analytical Columns

Connections (Physical):
Connector(s): 10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions:
Channel Size: 1.3 µm
Diameter: 5.2 mm
Length: 4.95 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 15 MPa | 150 bar | 2175 psi
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)
pH Stability: Working range 2–13, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 0.1 mL
Ligand: QA (strong anion exchanger, quaternary amine)
Ligand Density: 0.45–0.65 mmol/mL wet support
Typical Dynamic Binding Capacity 10% per mL: 25–31 mg BSA/mL wet support, BSA, 0.5 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flowrate 1 mL/min

Product Information:
Brand: CIMac™