Sartorius, 25896-010, Blue Trisacryl® M - 100 mL

Blue Trisacryl® M is an affinity resin that is used for the purification of enzymes, interferons, and some coagulation factors, as well as for the removal of albumin. The resin uses a Trisacryl base matrix which carries the blue dye ligand.
Bulk resins offer flexibility and scalability for large-scale bioprocessing
Allow for customized column packing to meet specific process requirements

Blue Trisacryl® M dye-affinity chromatography resin is used for the purification of a wide variety of enzymes and proteins such as kinases, interferons, and some coagulation factors. It is also used for albumin purification, or albumin depletion in samples for proteomics research.
Blue Trisacryl® M resin is based on Trisacryl, a macroporous non ionic synthetic polymer on which blue dye is covalently immobilized. The blue dye interacts biospecifically with some proteins due to its structural similarity with nucleotide cofactors (ADP, NADP). Other proteins like albumin or interferon bind by a combination of hydrophobic, electrostatic and pi-pi interactions.
Bulk resins offer adaptable scalability for large-scale bioprocessing, allowing tailored column packing to efficiently address specific purification requirements.
Features and Benefits
Unique multi-modal interaction mechanism
Low ligand leakage
Multiple applications from laboratory to production scale
Applications
Enzymes which need NAD as cofactor (kinases, dehydrogenases, phosphatases)
Other enzymes (sulfatases, RNA polymerases, mono-oxygenases, oxidoreductases)
Albumin from plasma
Transferin from plasma
α1-acid glycoprotein, α-fetoprotein, α1-proteinase inhibitor
Serine proteases
Interferons
DNA antibodies
Recombinant vaccines purification processes

Specifications
Applications:
Application: Preparative protein purification

Compliance Information:
Quality Standards: CoA; RSF (upon request)

Dimensions:
Bead Size: 40-80 µm

General Specifications:
Flow Rate: 100-150 cm/h
Resin Format: Bulk resin

Materials of Construction:
Base Matrix: Synthetic Polymer

Operating Conditions:
Operating Pressure: < 3 barg (44psi)
pH Stability: 1 to 12 (short term), 4 to 10 (long term)

Physicochemical Information:
Ligand: Blue dye
Reference Protein: HSA
Typical Dynamic Binding Capacity 10% per mL: >10 mg/mL HSA

Product Information:
Brand: Blue Trisacryl® M
Pack Size: 1
Pack Volume: 100 mL
Resin Type: Affinity resin
What you receive: 1 bottle resin; Instruction for use

Storage Conditions:
Storage Conditions: 20% EtOH/1M NaCl