Sartorius, 411.5114-6, CIMmultus® DEAE 8 mL Monolithic Column (6 μm)

This high-performance ion exchange column is primarily intended for fast and efficient purification of biomolecules, especially for plasmid DNA, through their ionic interaction with the monolith.

CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production. CIMmultus® can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.
CIMmultus® DEAE is a high-performance ion exchange column, primarily intended for fast and efficient purification of biomolecules, especially for plasmid DNA, through their ionic interaction with the monolith.
DEAE features:
Weak anion exchanger
Working range, pH 2–9
Binds molecules with a predominantly negative charge, repels molecules with a predominantly positive charge
Use for capture, intermediate, or polishing steps in either bind-elute or flow-through mode
Benefits:
Very fast processes by convective mass transport – resolution independent of flow
Ready to use columns
High-capacity for large molecules due to fully accessible void volume in the channels
Laminar flow minimizes shear stress resulting in high yields

Specifications
Applications:
Application: Preparative Applications

Compliance Information:
GMP Compliance: non-cGMP

Connections (Physical):
Connector(s): 10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions:
Channel Size: 6 µm
Column Tube Length: 56 mm
Inner Diameter: 6.5 mm
Outer Diameter: 15 mm

Features:
Reusable: Yes

General Specifications:
Flow Rate: 0.8–100 mL/min | 2.4–335 cm/h | 0.1–12.5 CV/min

Materials of Construction:
Materials of Construction: Poly(glycidyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions:
Maximum Operating Pressure: 1.4 MPa | 14 bar | 200 psi
Recommended Operating Temperature: 4 °C (39 °F) to 40 °C (104 °F)
pH Stability: Working range 2–10, cleaning in place 1–14

Physicochemical Information:
Bed Volume: 8 mL
Ligand: DEAE (weak anion exchanger, diethylamino)
Typical Dynamic Binding Capacity 10% per mL: ≥ 5 mg BSA/ml wet support, BSA, 1.0 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flow rate 16

Product Information:
Brand: CIMmultus®
Package Type: Individually Packed

Volume:
Volume: 8 mL