Sartorius, 93IDA-42DB-12--V, Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

Top Features of Sartobind® Lab IDA Metal Affinity Membrane Adsorbers
Achieve optimum his-tagged protein purity and yield, using the metal ion of your choice
Higher flow rates compared with metal affinity resins
Operate by syringe, peristaltic pump or FPLC system
Popular Applications for Sartobind® Lab IDA Metal Affinity Membrane Adsorbers

Based on the Patented Sartobind® Platform
Sartobind® Lab membrane adsorbers for immobilized metal affinity chromatography (IMAC) are ready-to-use units in a syringe filter format. They are based on the patented macroporous Sartobind® membranes, with iminodiacetic acid (IDA) covalently bound to the entire internal surface of the pores. This results in a chromatography matrix with superior flow rates and high binding capacity. The IDA ligand enables coupling of the metal ion of your choice to the membrane adsorber, ensuring simple process optimization for maximum purity and yield of each his-tagged protein.
Ultimate Versatility
Sartobind® Lab IDA units are available with a 2.1 mL bed volume, providing binding capacities up to 7.5 mg his-tagged protein per unit. Equipped with standard Luer lock connectors, Sartobind® Lab units enable simple, equipment-free handling by syringe, or can be easily connected to FPLC systems for automated purifications. The units are compatible with most common chromatography buffers and can be regenerated for multiple purification cycles, ensuring high-cost efficiency.

Specifications
Applications:
Application: Screening; Optimization; His-tagged Protein Purification
Intended Use: Research Use Only (RUO)

Dimensions:
Bed Height: 4 mm
Membrane Area: 75 cm²

Features:
Reusable: Yes

Materials of Construction:
Housing Material: Polypropylene (PP)
Membrane Material: Stabilized reinforced cellulose
Reinforcement Material: Polyester

Operating Conditions:
Maximum Operating Pressure: 0.6 MPa
Recommended Flow Rate: 10-63 mL/min (5-30 MV/min)
pH Stability: pH 1 – 12 (short term), pH 3 – 9 (long term)

Physicochemical Information:
Bed Volume: 2.1 mL
Chemical Compatibility: Stable in common chromatography buffers, avoid peroxide and other oxidizing or reactive reagents
Ligand: Iminodiacetic acid (IDA)
Ligand Density: 5 µeq/cm²
Pore Size: 3 - 5 µm
Recommended Chelating Ions: Ni2+, Co2+, Cu2+, or Zn2+
Reference Protein: Polyhistidine tagged protein
Typical Dynamic Binding Capacity 10% per unit: 7.5 mg
Void Volume: 1.3 mL

Product Information:
Brand: Sartobind® Lab
Membrane Type: Metal chelate
Pack Size: 2
What you receive: 2 units; 1 operating instruction sheet