Sartorius, PRC08X100PPAHCEL, PPA HyperCel PRC Column - 5 mL

PPA HyperCel is a Mixed Mode resin with an aromatic ligand exhibiting stronger hydrophobicity and providing a different selectivity in protein purification.
Pre-packed columns simplify resin screening and selection.
Ideal for process step optimization
RoboColumn® format is especially suited for high throughput process development (HTPD)

PPA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a phenlypropylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. PPA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).
PPA HyperCel pre-packed columns are designed to simplify resin screening and selection, making them ideal for optimizing process steps. The RoboColumn® format is particularly suited for high throughput process development (HTPD), allowing for efficient and streamlined purification processes.
Features and Benefits
High mechanical and chemical stability
Suitable for large-scale chromatography in a biopharmaceutical environment
Cross-linked cellulose base matrix
Applications
Protein purification at low ionic strength by direct hydrophobic capture
Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
Orthogonal separation mechanism to ion exchange or HIC

Specifications
Applications:
Application: Resin screening and process optimization

Compliance Information:
Quality Standards: CoA; RSF

Dimensions:
Bead Size: 80 - 100 µm
Bed Height: 100 mm
Dimensions: 8 x 100 mm

General Specifications:
Flow Rate: 200-600cm/h
Resin Format: Pre-packed column

Materials of Construction:
Base Matrix: Rigid cellulose beads
Materials of Construction: Polypropylene

Operating Conditions:
Operating Pressure: < 3 barg (44psi)
pH Stability: 1 to 14 (short term), 2 to 12 (long term)

Physicochemical Information:
Bed Volume: 5 mL
Ligand: Phenylpropylamine
Reference Protein: Bovine serum albumin (BSA)
Typical Dynamic Binding Capacity 10% per mL: 40-60 mg/mL BSA

Product Information:
Brand: PPA HyperCel
Pack Size: 1 column per pack
Pack Volume: 5 mL
Resin Delivery Condition: 24% iPrOH/100mM sodium phosphate, pH 4.3
Resin Type: Mixed Mode anion exchanger
What you receive: 1 column, instructions

Storage Conditions:
Storage Conditions: 20% EtOH/1M NaCl