Thermo Fisher, 0010057AE, Anti-Clumping Agent

Thermo Fisher, 0010057AE, Anti-Clumping Agent GIBCO™ Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.
• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin-free and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and deliverySimple and Gentle Cell DissociationCell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping” state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.
Reduced Clumping, Increased Viability, Improved Protein ExpressionReduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.
For actual cell dissociation, we recommend our animal origin-freeTrypLE™ products.
Use Anti-Clumping Agent after TransfectionAnti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.
Animal Origin-free and Chemically Defined ComponentsBecause Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.
Convenient and Customizable PackagingPreviously, Anti-Clumping Agent was only available in a 20 mL bottle. However, it is now available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking on the 'Customize this Product' link under the 'Add to Cart' button above or by emailing us atCustomMedia@lifetech.
com.
Additional Information and SupportPlease review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to ourTechnical Servicesgroup.
For Research Use Only. Not for use in diagnostics procedures.
Cell Line: CHO, Insect Cells, 293 (HEK)
Cell Type: Mammalian Cells
Culture Type: Suspension Cell Culture
Format: Bottle(s)
Product Line: Gibco™
Quantity: 20 mL
Shipping Condition: Room Temperature
Source: Animal origin-free
Classification: Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Form: Liquid
Product Type: Anti-clumping Reagent
pH: 6 to 8
Unit Size: Each