Thermo Fisher, 10372019, MCDB 131 Medium, no glutamine Gibco™

MCDB 131 Medium, no glutamine Gibco™ MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.
This MCDB 131 Medium is manufactured as follows:WithWithout
• Phenol Red
• L-glutamine
• Sodium pyruvate
• HEPESThe completeformulationis available.
Using MCDB 131MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels ofFetal Bovine Serum(FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.
176 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.
Dual-site cGMP manufacturingFor supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
Cell Type: Human Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells
Concentration: 1 X
Product Line: Gibco™
Product Type: MCDB 131 Medium
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Culture Type: Mammalian Cell Culture
Form: Liquid
Sterility: Sterile-filtered
With Additives: Low Glucose, Phenol Red, Sodium Pyruvate
Without Additives: No Glutamine, No HEPES
Unit Size: Each