Thermo Fisher, 10486025 , Express Five™ SFM 

Express Five™ SFM  Express Five™ SFM is a protein-free, serum-free medium specifically developed to support the long-term suspension growth of High Five™ (BTI-TN-5B1-4) cells in suspension for use in the Baculovirus Expression Vector System (BEVS). This medium requires the addition of 18 mM L-glutamine before use. Gibco™ Express Five™ SFM features:
• Superior long-term suspension growth of High Five™ cells
• Protein-free, serum-free formulation
• Optimized for recombinant protein production
• Scalable in the CelliGen™ bioreactor and WAVE Bioreactor™ SystemSuperior growth of High Five™ cellsGibco™ Express Five™ SFM provides peak cell densities of up to 6 × 106viable cells per mL in shaker cultures. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco™ Express Five™ SFM, usually without any further adaptation (see figure). Cells usually require adaptation from serum-containing formulations.
Protein-free, serum-free formulationGibco™ Express Five™ SFM is a protein-free and serum-free formulation, allowing for easier purification of your protein of interest.
Optimized for recombinant protein expressionGibco™ Express Five™ SFM is optimized for high protein production using the Baculovirus Expression Vector System (BEVS). Using BEVS, we routinely achieve rβ-galactosidase protein expression of up to 1.
7 U/cell, a significant improvement over other media (see figure)Scalable in the CelliGen™ bioreactor and WAVE Bioreactor™ SystemProtein production using Gibco™ Express Five™ SFM can be scaled up in bioreactors such as the 5 L CelliGen™ bioreactor and the WAVE Bioreactor™ System. The appropriate impeller or rocking speed and seeding density should be optimized for each system. Depending on the bioreactor design, it may be necessary to supplement Gibco™ Express Five™ SFM with additional Pluronic™ F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality systemGibco™ Express Five™ SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Cell Line: High Five™
Cell Type: Insect Cell
Product Line: Express Five™, Gibco™
Product Type: Insect Cell Serum Free Medium (SFM)
Quantity: 1000 mL
Shipping Condition: Room Temperature
Classification: Protein-free, Serum-free
Form: Liquid
Serum Level: Serum-free
Without Additives: No Glutamine
Unit Size: Each