Thermo Fisher, 10503027, DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples

DNAzol™ Reagent, for isolation of genomic DNA from solid and liquid samples DNAzol™ Reagent is a complete and ready-to-use organic reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol™ Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–100%. DNAzol™ Reagent is:
•Versatile—can use with a broad spectrum of sample materials
•Efficient—rapid isolation and high recovery of genomic DNAEfficient isolation of genomic DNA from a variety of sample typesThe DNAzol™ Reagent procedure uses a novel guanidine-detergent lysing solution that permits selective precipitation of DNA from cell lysate. 1 mL of DNAzol™ Reagent can be used to isolate genomic DNA from 1–3 x 107cells, from 0.
1 mL of whole blood, or from 25–50 mg tissue.
Rapid isolation and high recovery of genomic DNAThe DNAzol™ Reagent combines reliability, efficiency, and simplicity in its DNA isolation protocol. During isolation, a biological sample is lysed (or homogenized) in DNAzol™ Reagent, and the genomic DNA is then precipitated from the lysate with ethanol. Following an ethanol wash, DNA may be solubilized in either water or 8 mM NaOH. The entire procedure can be completed in 10–30 min with DNA recovery of 70–100%.
Isolated DNA can be used for a number of downstream applicationsGenomic DNA extracted using the DNAzol™ procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.
Elution Volume: Varies
Final Product Type: Genomic DNA
For Use With (Application): PCR, southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 100 mL
Sample Type: Bacteria, Cells, Plant, Tissue, Yeast
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤107cells or 0.
1 mLCells: ≤107Plant: ≤50 mgTissue: ≤50 mgYeast: ≤107cells or 0.
1 mL
Test Time: 30 min.
Isolation Technology: Organic Extraction
Unit Size: Each