Thermo Fisher, 10565018, DMEM/F-12, GlutaMAX™ supplement Gibco™

DMEM/F-12, GlutaMAX™ supplement Gibco™ DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This DMEM / F-12 is modified as follows:WithWithout
• GlutaMAX
• HEPES
• Phenol RedThe completeformulationis available.
Using DMEM/F-12DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 withGlutaMAXsupplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10%Fetal Bovine Serum(FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Line: MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™, GlutaMAX™
Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
Without Additives: No HEPES
Unit Size: Each