Thermo Fisher, 10978021, Plant DNAzol™ Reagent

Plant DNAzol™ Reagent Plant DNAzol™ Reagent is a ready-to-use organic reagent formulated for the isolation of high quality genomic DNA from a variety of plant samples.
• Permits use on broad spectrum plant material
• Rapid isolation of genomic DNARNA Hydrolysis for Efficient Isolation of Genomic DNA from a Variety of Sample SourcesPlant DNAzol™ Reagent can be used for the efficient isolation of genomic DNA from a variety of plant tissues such as leaf, seed stem, root, and callus. The Plant DNAzol™ procedure is based on the use of a novel guanidine-detergent lysing solution that hydrolyzes RNA and allows the selective precipitation of DNA from the lysate.
Isolate Genomic Plant DNA in About an HourIn the Plant DNAzol™ procedure, plant samples are pulverized in liquid nitrogen or homogenized, and genomic DNA is extracted from the homogenate with the Plant DNAzol™ Reagent. Following extraction, plant debris is removed by centrifugation and DNA is precipitated from the supernatant with ethanol, and solubilized withTE buffer (pH 8.
0). The entire procedure can be completed in approximately 60-70 minutes.
Comparable Yield in Less Time and with Fewer ReagentsUnlike traditional methods for plant DNA extraction (e.
g., CTAB), Plant DNAzol™ is quicker, does not require RNase A to remove RNA from genomic DNA preparations, and in the majority of tissues, does not require phenol. Furthermore, use of Plant DNAzol™ generates yields from plant tissue that are comparable with the traditional CTAB method.
Isolated DNA can be used for a Number of Downstream Applications:
•Amplified Fragment Length Polymorphism (AFLP)
•Restriction Fragment Length Polymorphism (RFLP)
•Southern Blotting
•Sequencing
•PCR
•CloningFor Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Elution Volume: 70 μL
Final Product Type: Genomic DNA
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 100 mL
Sample Type: Plants
Shipping Condition: Room Temperature
Starting Material Amount: 0.
1 g
Test Time: 60 min.
Yield: ≤300 μg
Isolation Technology: Organic Extraction
Unit Size: Each