Thermo Fisher, 11330057, DMEM/F-12, HEPES

DMEM/F-12, HEPES Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This DMEM / F-12 is modified as follows:With:L-glutamine, HEPES, Phenol RedThe completeformulationis available.
Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10%Fetal Bovine Serum(FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2environment to maintain physiological pH.
Cell Line: MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Concentration: 1 X
For Use With (Application): Mammalian cell culture
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: DMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity: 10 x 500 mL
Shelf Life: 12 Months From Date of Manufacture
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
Unit Size: Each