Thermo Fisher, 11441D, Dynabeads™ Mouse Pan B (B220)

Dynabeads™ Mouse Pan B (B220) Dynabeads™ Mouse pan B (B220) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse B cellsExcellent Yield, Purity, and Viability of Recovered CellsDynabeads™ Mouse pan B (B220) are uniform, superparamagnetic beads (4.
5 μm diameter) that enable easy isolation of mouse B cells from spleen or lymph nodes, although any single cell suspension may be used. Dynabeads™ Mouse pan B beads are coated with a primary monoclonal antibody specific for the B220 membrane antigen. B220 (also known as CD45R) is expressed on all B cells throughout development, from the early pro-B stage of B cell differentiation. Dynabeads™ Mouse pan B binds to B cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using amagnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated B cells.
Ideal for Both Depletion and Positive Isolation for Downstream Molecular AssaysBecause of the powerful magnets used for separation, Dynabeads™ Mouse pan B (B220) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable the efficient depletion of mouse B cells in about 30 minutes. Dynabeads™ Mouse pan B will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that viable cells will not be released from these beads, so if you want to isolate mouse B cells for cell-based applications, or you need to check your samples with flow cytometry, you should useDynabeads™ Mouse CD43 (Untouched™ B cells).
Select what is right for you from our range ofMouse B cell Isolation Products.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Cell Type: B Cells
Isolation Technology: Depletion, positive isolation for molecular applications
No. of Cells: Processes ∼2 x 109cells total
Output Viability: >95%
Product Line: DYNAL™, Dynabeads™
Purity or Quality Grade: Research Grade
Quantity: 5 mL
Reactivity: Mouse
Sample Type: Lymph Node, Blood, Spleen
Shipping Condition: Room Temperature
Starting Material Cell No.: 1 x 107cells per isolation
Target Species: Mouse
Diameter (Metric): 4.
5 μm
Product Type: Antibody Coated Bead
Unit Size: Each