Thermo Fisher, 11900016, MEM α, nucleosides, powder

Quantity: 1 L Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This MEM α is modified as follows:WithWithout
• Ribonucleosides
• Sodium Bicarbonate
• Deoxyribonucleosides
• L-glutamine
• Phenol RedThe completeformulationis available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). MEM α uses a sodium bicarbonate buffer system (2.
2 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (seeprotocolfor details).
Cell Type: Mammalian Cells
Culture Environment: CO2
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Osmolality: 240 - 290 mOsm/kg
Product Line: Gibco™
Product Type: Minimum Essential Medium (MEM) alpha
Quantity: 1 L
Shelf Life: 36 Months
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Culture Type: Mammalian Cell Culture
Form: Powder
Serum Level: Standard Serum Supplementation
With Additives: Glutamine, Phenol Red, Sodium Pyruvate
Without Additives: No HEPES, No Sodium Bicarbonate
pH: 3.
3 to 3.
9
Unit Size: Each