Thermo Fisher, 12183555, TRIzol™ Plus RNA Purification Kit

TRIzol™ Plus RNA Purification Kit The TRIzol Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol Reagent (Cat. No. 15596026), followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink RNA Mini Kit (Cat. No. 12183020), to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues.
Features of this kit include:
• Combined TRIzol-based lysis and PureLink spin column-based RNA isolation technologies
• Superior lysis capability and convenient protocolTwo products in oneThe TRIzol Plus RNA Purification System couples the lysis capabilities of TRIzol Reagent with the 1000 μg column-;binding capacity of the PureLink RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.
Simple lysis to column purification protocolTRIzol Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.
5 may also be used).
Elution Volume: 30 to 300 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 1 hr.
Quantity: 50 Preps
Scale: Mini
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤107Cells: ≤107Plant: ≤107, ≤100 mgTissue: ≤100 mgYeast: ≤107
Yield: Up to 350 μg
Isolation Technology: Silica Spin Column, Organic Extraction
Sample Type: Bacteria, Cells, Plant Samples, Tissue, Yeast
Unit Size: Each