Thermo Fisher, 12276010, Gateway™ pET-DEST42 Vector

Gateway™ pET-DEST42 Vector The Champion™ pET-DEST42 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3)E. coli. The Champion™ pET-DEST42 destination vector offers:
• Bacteriophage T7lacpromoter for high-level expressionO operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purificationR sites for efficient recombination with anyattL-flanked Gateway™ entry vectorAntibiotic Resistance Bacterial: Ampicillin (AmpR)
Constitutive or Inducible System: Inducible
Inducing Agent: IPTG
Product Type: Gateway System Destination Expression Vector
Quantity: 6 μg
Selection Agent (Eukaryotic): None
Vector: pET, pDEST
Cloning Method: Gateway
Product Line: Gateway™
Promoter: T7, lacO
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each