Thermo Fisher, 12604039, TrypLE™ Express Enzyme (1X), no phenol red

TrypLE™ Express Enzyme (1X), no phenol red TrypLE Express is an animal origin-free, recombinant enzyme used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. We offer a variety ofTrypLE formulationsfor a range of cell culture applications. Compared to trypsin and other dissociation reagents, TrypLE Express is:
• Gentle on cells
• Room temperature-stable
• Easy to use
• Animal origin-free (AOF)This TrypLE Express is modified as follows:WithWithout
• EDTA
• Phenol RedTheformulationof TrypLE solutions is available, although we keep the enzyme concentration confidential.
Gentle on cellsTrypLE Express' exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room temperature-stableTrypLE Express remains stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to useTrypLE Express can be directly substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS.
Animal origin-free (AOF)Unlike porcine trypsin, TrypLE Express is free of animal-derived components.
cGMP manufacturing and quality systemFor supply chain continuity, we manufacture Gibco TrypLE Express Enzyme (1X), no phenol red at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
Cell Type: Mammalian Cells
Chelators: EDTA
Concentration: 1 X
Culture Type: Adherent Cell Culture
Green Features: Energy efficient, Sustainable packaging
Osmolality: Check and Record
Product Line: Gibco™
Quantity: 20 x 100mL
Remarks: Room Temperature Stable
Shelf Life: 24 Months
Shipping Condition: Room Temperature
Source: Animal origin-free
Tested For: In vitro Bioassay
Classification: Animal Origin-free
Form: Liquid
Product Type: Cell Culture Dissociation Reagent
Sterility: Sterile-filtered
Without Additives: No Phenol Red
pH: 6 to 8
Unit Size: Each