Thermo Fisher, 15367014, Plasmid pBR322

Plasmid pBR322 Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™E. coli, also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites forHind III andClaI are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance.
Performance and Quality Testing:Purity, DNA structure, and selected restriction endonuclease fragmentation patterns are verified by agarose gel analysis. Transformation ofE. coliHB101 to confirm AmpR and TetR is confirmed by plating on selective medium.
Cloning Method: Restriction Enzyme/ MCS
Concentration: 0.
25 μg/μl
For Use With (Application): Cloning
Product Type: Plasmid
Quantity: 25 μg
Shipping Condition: Approved for shipment on Wet or Dry Ice
Vector: pBR322
Unit Size: Each