Thermo Fisher, 15529019, IPTG Invitrogen™

IPTG Invitrogen™ IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detectlacgene activity in cloning procedures. IPTG binds to thelacrepressor, releasing it from thelacoperator, and thereby allowing the transcription of genes in thelacoperon, such as the gene coding for β-galactosidase.
In addition, IPTG is one of the most efficient molecular inducers for promoter’s transcriptional activity and is used extensively for heterologous protein expression induction inE. coli. Though mainly used in cell culture assays, publications demonstrated the use IPTGin vivoin animal models too.
Primary applications
• Blue-white screening—induce functional β-galactosidase
• Induce protein expression from inducible T7 RNA polymeraseE. coliexpression systems egulated by lac operonFind the media format that fits your needsWe offer a wide variety of ready-to-use media formulations, such asLB Broth,Terrific BrothorS.
O.
C recovery medium.
X-GalorBluo-Galfor blue-white screening.
Explore other media formats forbacterial growth.
Chemical Name or Material: IPTG (Isopropyl β-D-thiogalactopyranoside)
Quantity: 1 g
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Form: Powder
Format: Bottle
Unit Size: Each