Thermo Fisher, 16154, pTK-Green Renilla Luc Vector for Luciferase Assays

pTK-Green Renilla Luc Vector for Luciferase Assays The Thermo Scientific pCMV-GreenRenillaLuc vector is a derivative of pMCS-GreenRenillaLuc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Green Renilla Luc vector:
• Weak (TK) constitutive promoter for co-transfection and normalization
• Intracellular GreenRenillaluciferase gene, optimized for high expression in mammalian systems
• Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
• Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
• High-copy pUC bacterial DNA replication originMore Product Data
•Luciferase assays in hard-to-transfect Jurkat cellsCloning Method: Restriction Enzyme/MCS
Delivery Type: Transfection
Description: pTK-Green Renilla Luc Vector
For Use With (Application): Reporter Assays
Product Line: Pierce™
Quantity: 10 μg
Reporter Gene: Green Renilla Luciferase
Selection Agent (Eukaryotic): Puromycin
Promoter: TK
Type: Luc Vector
Unit Size: Each