Thermo Fisher, 16165, Pierce™ Renilla Luciferase Flash Assay Kit

1000 Reaction Kit The Thermo Scientific PierceRenillaLuciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.
Features of theRenillaLuciferase Flash Assay Kit:
•Sensitive—greater sensitivity allows utilization of smaller numbers of cells
•Cost effective—highest sensitivity assays result in decreased reagent consumption
•Time-saving—assays require minimal sample handling
•Automation-friendly—amenable to high throughput screens
•Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
•Safe—allows one to perform non-radioactive assaysThis Flash Assay Kit contains reagents for measuring the activity ofRenillaluciferase in mammalian cell lysates. When used with Thermo Scientific GreenRenillaLuc Plasmids, the kit provides an extremely sensitive bioluminescent reporter assay system for intracellular detection of promoter or pathway activity. The signal produced by GreenRenillaLuc is considerably greater than signal from either native firefly orRenillaluciferases assayed under similar conditions. Light output captured using a luminometer can be correlated with the amount ofRenillaluciferase protein produced and used to determine the activity of the promoter drivingRenillaexpression.
Includes:Cell lysis buffer, reaction buffer and substrateRequires:Renillaluciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
Applications:
• Promoter studies for analyzingcis- regulatory elements andtrans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studiesThe GreenRenillaluciferase is a 36kDa protein produced by a derivative of the wild typeRenillaluciferase gene from the sea pansy,Renilla reniformis. Compared to the wild type luciferase, GreenRenillais more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The PierceRenillaFlash Assay Kit reagent optimizes the this signal for use in flash-type luciferase reporter assays. The kit also is compatible for flash assays with otherRenillaluciferases and derivatives that use coelenterazine as a substrate.
More Product Data
•Luciferase assays in hard-to-transfect Jurkat cells
•Versatile luciferases: microplate luminometers and flash luciferase assaysAssay: Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescence
For Use With (Equipment): Luminometer (Microplate)
Format: 384-well plate, 96-well plate
Label Type: Enzyme Labeled
Product Line: Pierce™
Quantity: 1000 Reaction Kit
Substrate: Coelenterazine
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: Luciferase, Green Renilla Luciferase
Technique: Enhanced Chemiluminescence
Type: Assay Kit
Unit Size: Each