Thermo Fisher, 16166, Pierce™ Renilla Luciferase Glow Assay Kit

100 Reaction Kit The Thermo Scientific PierceRenillaLuciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of GreenRenillaluciferase reporter.
Features of theRenillaLuciferase Glow Assay Kit:
•Sensitive—highly sensitive detection of GreenRenillaluciferase activity
•Stable—increased signal stability compared to flash assays
•Simple—does not require a luminometer with injectors
•Compatible—assay reagents compatible with otherRenillaluciferases
•Automation-friendly—amenable to high throughput assays
•Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
•Safe—allows one to perform non-radioactive assaysThis Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity ofRenillaluciferase in cell lysates. In the presence of luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific GreenRenillaLuc Plasmids; however, this glow kit may be used to detect the activity of otherRenillaluciferase derivatives that use coelenterazine as a substrate.
Includes:Cell lysis buffer, reaction buffer and substrateRequires:Renillaluciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
Applications:
• Promoter studies for analyzingcis- regulatory elements andtrans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studiesThe GreenRenillaluciferase is a 36kDa protein produced by a derivative of the wild typeRenillaluciferase gene from the sea pansy,Renilla reniformis. Compared to the wild type luciferase, GreenRenillais more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The PierceRenillaGlow Assay Kit reagent effectively stabilizes this bright bioluminescence to yield a signal half-life greater than 3 hours, thus eliminating the need for a luminometer with injectors. The assay kit was optimized for best results with Thermo Scientific GreenRenillaLuc Plasmids; however, this glow kit may be used to detect the activity of otherRenillaluciferase derivatives that use coelenterazine as a substrate.
Assay: Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescence
For Use With (Equipment): Luminometer (Microplate)
Format: 384-well plate, 96-well plate
Label Type: Enzyme Labeled
Product Line: Pierce™
Quantity: 100 Reaction Kit
Substrate: Coelenterazine
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: Luciferase, Green Renilla Luciferase
Technique: Enhanced Chemiluminescence
Type: Assay Kit
Unit Size: Each