Thermo Fisher, 16177, Pierce™ Firefly Luciferase Glow Assay Kit

1000 Reaction Kit The Thermo Scientific Pierce Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.
Features of the Firefly Luciferase Glow Assay Kit:
•Sensitive—highly sensitive detection of firefly luciferase activity; for best results (maximum signal) use withPierce Firefly Luciferase Signal Enhancer
•Stable—increased signal stability compared to flash assays
•Simple—does not require a luminometer with injectors
•Compatible—assay reagents compatible with other firefly beetle luciferases
•Automation-friendly—amenable to high throughput assays
•Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
•Safe—allows one to perform non-radioactive assaysThis Pierce Luciferase Glow Assay Kit contains reagents for measuring the activity of firefly luciferase in cell lysates. In the presence of firefly luciferase, the glow reagent provides stable bioluminescent signal and is highly suited for luminometers without injectors or for batch processing of samples. The light output generated by the luciferase reaction can be correlated with the amount of luciferase protein produced which in turn is proportional to the promoter activity driving the luciferase expression. The glow assay reagents were optimized for best results with Thermo Scientific Firefly Luc Plasmids; however, this assay kit may be used to detect the activity of other firefly or ATP-dependent luciferases that use D-luciferin as a substrate.
Includes:Cell lysis buffer, reaction buffer and substrate; purchaseLuciferase Signal Enhancerseparately.
Requires:
• Firefly luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.
• For best results (maximum signal), use withPierce Firefly Luciferase Signal EnhancerApplications:
• Promoter studies for analyzingcis- regulatory elements andtrans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studiesFirefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the generaPhotinusandLuciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output generated by the luciferase reaction is captured using a luminometer and can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression. The native firefly luciferase produces a strong flash signal that decays rapidly. The signal produced using the Pierce Firefly Glow Assay Kit is more stable and decays very slowly, thereby eliminating the need for a luminometer with injectors.
Assay: Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescence
For Use With (Equipment): Luminometer (Microplate)
Format: 384-well plate, 96-well plate
Label Type: Enzyme Labeled
Product Line: Pierce™
Quantity: 1000 Reaction Kit
Substrate: D-Luciferin
Substrate Properties: Chemical Substrate
Substrate Type: Luciferase Substrate
Target: Luciferase, Firefly Luciferase
Technique: Enhanced Chemiluminescence
Type: Assay Kit
Unit Size: Each