Thermo Fisher, 16600108, McCoy's 5A (Modified) Medium

10 x 500 mL McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:WithWithout
• High Glucose
• Sodium Pyruvate
• L-glutamine
• HEPES
• Bacto-peptone
• Phenol RedThe completeformulationis available.
Using McCoy's 5A (modified) MediumDr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10%Fetal Bovine Serum(FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.
2 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Line: Rat fibroblasts
Cell Type: Biopsy Tissues
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: McCoy's 5A Modified Medium
Quantity: 10 x 500 mL
Shelf Life: 12 Months From Date of Manufacture
Classification: Animal Origin
Form: Liquid
Sterility: Sterile-filtered
Sterilization Method: Sterile-filtered
With Additives: High Glucose, Glutamine, Phenol Red, Bacto-peptone
Without Additives: No HEPES, No Sodium Pyruvate
Unit Size: Each