Thermo Fisher, 18080400, SuperScript™ III First-Strand Synthesis SuperMix

SuperScript™ III First-Strand Synthesis SuperMix The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 0.
1 pg to 5 μg of total RNA.
This kit includes SuperScript III/RNaseOUT enzyme mix, 2X First-Strand reaction mix, and an annealing buffer. SuperScript III Reverse Transcriptase is a version of MMLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than many previous reverse transcriptases.
Using SuperScript III First-Strand Synthesis SuperMixSuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA. RNaseOUT Recombinant RNase Inhibitor is included in the enzyme mix to safeguard against degradation of target RNA due to ribonuclease contamination. The 2X First-Strand reaction mix includes 10 mM MgCl2and 1 mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA. The annealing buffer is used in the initial template-primer annealing step. Separate tubes of oligo(dT)20and random hexamers are also provided. cDNA synthesis can be performed using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer.
Final Product Type: First-Strand cDNA
Format: Kit
No. of Reactions: 50 Reactions
Optimal Reaction Temperature: 50°C
Primer: Random Primers, Oligo dT Primers
Quantity: 50 rxns
Reaction Format: Master Mix
Reagent Type: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Starting Material: RNA
Technique: Reverse Transcription
For Use With (Application): Real Time PCR (qPCR)
GC-Rich PCR Performance: High
Reaction Speed: 50 min.
Unit Size: Each