Thermo Fisher, 18094011, BioPrime™ DNA Labeling System

BioPrime™ DNA Labeling System The BioPrime™ DNA Labeling System is specifically designed for use in the preparation of biotinylated probes. Random primers (octamers) are annealed to the denatured DNA template and extended by Klenow fragment in the presence of biotin-14-dCTP to produce sensitive biotinylated-DNA probes for use in the nonradioactive detection of DNA and RNA. With the BioPrime™ DNA Labeling System, considerable net DNA synthesis occurs resulting in a 10–40 fold amplification of the probe. This product is particularly well suited for situations in which the DNA is limited. Probes made with this product have been used for hybridization to Southern and northern blots, plaque and colony lifts, and forin situhybridization to chromosome spreads. The BioPrime™ DNA Labeling System:
• Requires less template DNA than nick translation
• Amplifies template to provide an increased amount of biotinylated probe
• Labels 50 to 500 ng of template DNA in one reactionIncludes Label or Dye: Yes
Labeling Method: Direct Labeling
Product Line: BioPrime™
Product Type: DNA Labeling System
Quantity: 30 Reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice
Detection Method: Biotin-based
Final Product Type: Probes (Labeled DNA)
Labeling Target: DNA (General), Genomic DNA
Label or Dye: Biotin
Unit Size: Each