Thermo Fisher, 18097012, BioPrime™ Total Genomic Labeling Module

BioPrime™ Total Genomic Labeling Module The BioPrime™ Total Genomic Labeling Module is a complete genomic DNA labeling system designed for use in array comparative genomic hybridization applications (aCGH) that allows for better call rates with precious samples.
Note: The BioPrime™ Total Genomic Labeling Module does not include PurelinkTMGenomic DNA purification columns and purification buffers. TheBioPrime™ Total Genomic Labeling Systemdoes include these components.
The BioPrime™ Total Genomic Labeling Module offers users the following advantages:- Higher signal to noise with Alexa Fluor™ 3 and 5 dyes- Less channel bias due to novel reaction formulation- Widest range of input material (50 ng - 3 μg)- Simplified workflow with master mix formulationThis complete, all-inclusive kit is optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component-limited reaction allows for consistent, robust DNA yields of ∼ 8 μg with as little as 50 ng of genomic DNA input. An optimized dye-labeled nucleotide mix with new dNTP linkers, novel application-specific Alexa Fluor™ 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor™ 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. New master mix formulation streamlines reaction set-ups, increasing consistency. Complete kits are batch tested to assure quality in performance from lot to lot.
Contents and Storage:The BioPrime™ Total Genomic Labeling Module contains Alexa Fluor™ 3 and Alexa Fluor™ 5 2x reaction mix, Exo-Klenow fragment (40 U/ μl), 5 mM EDTA, TE Buffer, and control DNA (Salmon Sperm). Store at -80°C for long term storage. The 2X Reaction Mixes may be stored at +4°C for up to 4 weeks and should be protected from light. All components are guaranteed for 6 months when properly stored.
Includes Label or Dye: Yes
Labeling Method: Direct Labeling
Product Line: BioPrime™
Product Type: Total Genomic Labeling Module
Quantity: 30 Reactions
Detection Method: Fluorescence
Final Product Type: Probes (Labeled DNA)
Labeling Target: Genomic DNA
Label or Dye: Alexa Fluor 3, Alexa Fluor 5
Unit Size: Each