Thermo Fisher, 18263012, Library Efficiency™ DH5α Competent Cells

Library Efficiency™ DH5α Competent Cells Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are able to achieve transformation efficiencies of >1 x 108cfu/μg control DNA per 100 μL reaction.
Library Efficiency DH5α Competent Cells are recommended for routine cloning of genes into large plasmids and are suitable for difficult clone constructions, such as blunt-end ligations or cloning using limited amount of DNA.
The strain has thelacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries therecA1 mutation, which helps reduce the rate of recombination while propagating plasmid DNA, and theendA1 mutation that makes DH5α cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification. DH5α cells are capable of being transformed efficiently with large plasmids and can serve as a host for the M13mp cloning vectors if a lawn ofDH5αF′IQ, for example, is provided to allow plaque formation.
Library Efficiency DH5α Competent Cells offer:
• Transformation efficiencies of >1 x 108cfu/μg
•lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• Plasmid DNA maintained with minimum recombination events due torecA1 mutation
•endA1 mutation that enables increased plasmid yield and quantity
•hsdR17 genotype that allows efficient transformation of cloned unmethylated DNA from PCRGenotypeF-Φ80lacZΔM15 Δ(lacZYA-argF) U169recA1endA1hsdR17 (rk-, mk+)phoAsupE44 λ-thi-1gyrA96relA1Find the strain and format that fits your needsDH5α strain and its derivativesare available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats.
MAX Efficiency DH5αF´IQ Competent Cellsare available in chemically competent format.
DH5α-T1R competent cells are available inMultiShot formatfor high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: Library Efficiency™
Product Type: Chemically Competent Cells
Quantity: 5 x 200 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each