Thermo Fisher, 18265017, Subcloning Efficiency™ DH5α Competent Cells Invitrogen™

Subcloning Efficiency™ DH5α Competent Cells Invitrogen™ Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106cfu/μg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any general application where DNA for transformation is not limiting.
Features of Subcloning Efficiency DH5α Competent Cells
• Designed for general, low throughput, everyday use
• Standard laboratory strain useful for general cloning applicationsIdeal for routine subcloning proceduresSubcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors. 500 μL of chemically competent cells are pre-aliquoted in tubes for ten reactions using 50 μL. These cells yield >1 x 106transformants/μg control DNA per 50 μL reaction. Subcloning Efficiency DH5α Competent Cells are not suitable for the generation of cDNA libraries.
Flexible cloning capabilitiesBenefits of Subcloning Efficiency DH5α Competent Cells include:
•lacZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
•recA1 that ensures increased insert stability and prevents unwanted recombination
•endA1 that improves the yield and quality of plasmid DNA prepared from minipreps
• Supports replication ofM13mpvectors but not plaque formationGenotypeF-Φ80lacZΔM15 Δ(lacZYA-argF) U169recA1endA1hsdR17(rk-, mk+)phoAsupE44thi-1gyrA96relA1 λ-Genetic marker descriptionsFind the strain and format that you needWe offer other DH strains inchemically competentandelectrocompetent cellformats to meet your specific needs.
Strains are available for challenging cloning applications such aslibrary preparationorcloning unstable DNA.
SOC medium forE. colirecovery after transformation isavailable, as well as other ready-to-usebacterial growth formats.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: Subcloning Efficiency™
Product Type: Chemically Competent Cells
Quantity: 4 x 500 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: Subcloning Efficiency (106to 107cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each