Thermo Fisher, 18290015, ElectroMAX™ DH10B Cells

ElectroMAX™ DH10B Cells ElectroMAX DH10B Cells are electrocompetentE. colicells offering the highest transformation efficiencies of >1 x 1010cfu/μg plasmid DNA. The high transformation efficiencies make these cells ideal for cloning both prokaryotic and eukaryotic genomic DNA and efficient plasmid rescue from eukaryotic genomes.
ElectroMAX DH10B Cells are suitable for construction of gene banks, generation of cDNA libraries using plasmid-derrived vectors, and for situations when DNA is limited. They are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.
ElectroMAX DH10B Cells provide:
• High-efficiency transformation to maximize clones
• Enhanced genetic markers for cDNA or gDNA cloning capabilitiesElectroMAX DH10B Cell features
• Φ80lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
•mcrA genotypic marker andmcrBC,mrrdeletion that allow cloning DNA that contains methylcytosine and methyladenine
•endA1 mutation that enables increased plasmid yield and quantityGenotypeF-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74recA1endA1araD139Δ(ara,leu)7697galUgalK λ-rpsLnupGGet more detailed information about specificGenotypes and Genetic Markersof ourE. coliCompetent Cells.
Find the strain and format that you needDH10B cellsare available in both electrocompetent and chemically competent formats.
ElectroMax DH10B T1R CellsandMegaX DH10B T1RElectrocomp Cellsoffer the benefit of T1 and T5 phage resistance.
Note:A high-voltage electroporation apparatus capable of generating field strengths of 16 kV/cm is required.
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >20 kb
Preparing Unmethylated DNA: No
Product Line: ElectroMAX™
Product Type: Electrocompetent Cells
Quantity: 5 x 100 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each