Thermo Fisher, 18297010, MAX Efficiency™ DH10B Competent Cells

MAX Efficiency™ DH10B Competent Cells MAX Efficiency DH10B Competent Cells are highly efficientE. colisuitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency, making them ideal for generating plasmid libraries, an ability to take up and maintain large plasmids (seen with plasmids of 150 kb in size), and a lack of methylation-dependent restriction systems.
Benefits of MAX Efficiency DH10B Competent Cells
• Ability to maintain large DNA plasmids
• Resistant to the effects of ligase and ligase buffer and tolerant of small amounts of undiluted ligation reactions
• Suitable for the construction of cDNA or genomic DNA libraries using plasmid-derived vectorsUniversal strain foreveryday applicationsThe presence of the mcrA genotypic marker and the deletion of mcrBC and mrr makes this strain suitable for cloning DNA that contains methylcytosine and methyladenine. Therefore, genomic DNA, both prokaryotic and eukaryotic, can be cloned efficiently in DH10B.
Versatile DH10B genotype features
•lacZ∆M15 for blue/white screening of recombinant clones
• Elimination ofmcrA,mcrBC,mrr, andhsdRMS restriction systems to allow construction of more representative genomic libraries
• endA1 mutation to increase plasmid yield and quantityGenotypeF–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1endA1araD139 Δ(ara-leu)7697galUgalK λ–rpsL(StrR)nupGGenetic marker descriptionsFind the strain and format that fits your needDH10B Cellsare available in both electrocompetent and chemically competent formats.
MegaX DH10B T1RElectrocomp Cellshave the highest transformation efficiency (>3 x 1010cfu/μg plasmid DNA).
Multishot formatsare available for high throughput applications.
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: MAX Efficiency™
Product Type: Chemically Competent Cells
Quantity: 5 x 200 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each