Thermo Fisher, 19524016, Biotin-14-dATP Invitrogen™

Biotin-14-dATP Invitrogen™ Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids inin situand filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 μg of DNA by nick translation.
Performance and Quality Testing:Purity is evaluated by reverse-phase HPLC analysis.
Concentration: 0.
4 mM
Format: Liquid
Labeling Method: Direct Labeling
Product Type: Labeled Nucleotide
Quantity: 50 nmol
Shipping Condition: Approved for shipment on Wet or Dry Ice
Label or Dye: Biotin and Other Hapten
Unit Size: Each