Thermo Fisher, 21565, EGS (ethylene glycol bis(succinimidyl succinate)) Thermo Scientific™

EGS (ethylene glycol bis(succinimidyl succinate)) Thermo Scientific™ Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.
5.
Features of the EGS crosslinker:
•Reactive groups:NHS ester(both ends)
•Reactive towards:amino groups (primary amines)
• Water-insoluble (dissolve first in DMF or DMSO); compare toSulfo-EGS
• Membrane-permeable, allowing for intracellular crosslinking
• Crosslinks formed are reversible at pH 8.
5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGSProduct References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: Yes
Description: EGS
Form: Powder
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 456.
36
PEGylated: No
Product Line: Pierce™
Quantity: 1 g
Reactive Moiety: NHS Ester
Shipping Condition: Ambient
Solubility: DMF, DMSO
Spacer Arm Length: 16.
1 Å
Water Soluble: No
Chemical Reactivity: Amine-Amine
Cleavable: By Hydroxylamine
Crosslinker Type: Homobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Medium (10 to 30 Å)
Unit Size: Each