Thermo Fisher, 21581, Pierce™ BS(PEG)5, No-Weigh™ Format

100 mg BS(PEG)5 is a bis-succinimide ester-activated PEG compound used for crosslinking between primary amines (—NH2) in proteins and other molecules. TheN-hydroxysuccinimide ester (NHS) groups at either end of the PEG5 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds.
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
Features of BS(PEG)5:
•Reactive groups:NHS ester (both ends)
•Reactive towards:amino groups (primary amines)
• NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds
• Water-soluble; compare to non-pegylatedBS3 (also soluble)andDSS (water-insoluble)
• Membrane-impermeable, allowing for cell surface labeling
• Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
• Polyethylene glycol spacer arms help maintain conjugate solubility
• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Ideal for small molecule or peptide conjugationsAlternative Names for BS(PEG)n:
• Bis-NHS-dPEG
• NHS-PEG-NHS
• BS(PEO)5
• Bis(NHS)PEO5
• BS-PEO9
• dPEG NHS ester
• Bis-succinimide ester PEG 400Product ReferencesCrosslinker Application Guide -- search for recent literature references for this productBioconjugate Techniques, Academic Press (Part No. 20036)Cell Permeability: No
Description: BS(PEG)5
Form: Liquid
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 532.
5
PEGylated: Yes
Product Line: Pierce™
Quantity: 100 mg
Reactive Moiety: NHS Ester
Shipping Condition: Ambient
Solubility: Water
Spacer Arm Length: 21.
7 Å
Water Soluble: Yes
Chemical Reactivity: Amine-Amine
Cleavable: No
Crosslinker Type: Homobifunctional
Format: Standard, Single-use
Product Type: Crosslinker
Spacer: Medium (10 to 30 Å)
Unit Size: Each