Thermo Fisher, 22308, EMCS (N-ε-malemidocaproyl-oxysuccinimide ester)

EMCS (N-ε-malemidocaproyl-oxysuccinimide ester) Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.
4 angstroms).
Features of EMCS:
•Reactive groups:NHS esterandmaleimide
•Reactive towards: amino and sulfhydryl groups
• C6-homolog of aliphatic spacer series which includesAMAS,BMPS,GMBSand EMCS
• NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
• Maleimides react with -SH groups at pH 6.
5-7.
5, forming stable thioether linkages
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare toSulfo-EMCS
• Increased sphere of coupling vs. GMBS
• Short aliphatic spacer has low potential for eliciting an immune responseProduct References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: Yes
Description: EMCS
Form: Powder
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 308.
29
PEGylated: No
Product Line: Pierce™
Quantity: 50 mg
Reactive Moiety: Maleimide, NHS Ester
Shipping Condition: Ambient
Solubility: DMF, DMSO
Spacer Arm Length: 9.
4 Å
Water Soluble: No
Chemical Reactivity: Amine-Sulfhydryl
Cleavable: No
Crosslinker Type: Heterobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Short (<10 Å)
Unit Size: Each