Thermo Fisher, 22589, Pierce™ Sulfo-SANPAH, No-Weigh™ Format

50 mg Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide. NHS esters react efficiently with primary amino groups (-NH2) in pH 7–9 buffers to form stable amide bonds. The reaction results in the release of sulfo-N-hydroxy-succinimide. When exposed to UV light, nitrophenyl azides form a nitrene group that can initiate addition reactions with double bonds, insertion into C-H and N-H sites, or subsequent ring expansion to react with a nucleophile (e.
g., primary amines). Sulfo-SANPAH, supplied as a sodium salt, is useful for cell-surface protein crosslinking and possesses charged groups for water solubility to a concentration of 10 mM.
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging prevents the loss of reagent reactivity and contamination over time by eliminating the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
Features of Sulfo-SANPAH:
•Reactive groups:sulfo-NHS esterandnitrophenyl azide
•Reactive towards: amino groups and any nucleophile
• Non-cleavable
•N-Sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino) hexanoate
• Optimal photolysis occurs at 320-350 nm; minimizes damage to biomolecules by irradiation
• Water soluble; non-cleavableProduct References:Crosslinker Application Guide -- search for recent literature references for this productCell Permeability: No
Form: Powder
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 492.
4
PEGylated: No
Product Line: Pierce™
Quantity: 50 mg
Reactive Moiety: Sulfo-NHS Ester, Aryl Azide
Shipping Condition: Ambient
Solubility: Water
Spacer Arm Length: 18.
2 Å
Water Soluble: Yes
Chemical Reactivity: Amine-Nonselective
Cleavable: By Thiols
Crosslinker Type: Heterobifunctional
Format: Standard, Single-use
Product Type: Crosslinker
Spacer: Medium (10 to 30 Å)
Unit Size: Each