Thermo Fisher, 23250, Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible Thermo Scientific™

Pierce™ BCA Protein Assay Kit - Reducing Agent Compatible Thermo Scientific™ This BCA Protein Assay Kit is a reducing agent-compatible version of our popular Pierce BCA Protein Assay. The kit enables you to measure protein concentration in samples that contain the disulfide reducing agents.
Compare all available BCA protein assays ›Features of the BCA Protein Assay Kit—Reducing Agent Compatible include:
•Compatibility—assay samples that contain up to 5 mM DTT, 35 mM BME, or 10 mM TCEP
•BCA technology—only a slight modification of the standard BCA Protein Assay protocol (15-min incubation with Compatibility Reagent); no precipitation steps required
•Sample volume—requires only 25 μL (standard kit) or less than 10 μL (microplate kit) of sample
•Colorimetric—measure with a standard spectrophotometer or plate reader at 562 nm
•Excellent uniformity—exhibits less protein-to-protein variation than dye-binding methods
•Assay range—linear working range for BSA of 125 to 2000 μg/mLThe BCA Protein Assay Kit—Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, BME, or TCEP. This reducing agent-compatible BCA kit extends the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.
Related productsPierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible96-Well Plates for Pierce BCA-RAC AssayAssay: BCA Assay
Description: Pierce BCA Tube Protein Assay Kit, Reducing Agent Compatible (RAC)
For Use With (Application): Solution-based Detection, Absorbance
For Use With (Equipment): Spectrophotometer, Microplate Reader
Product Line: Pierce™
Product Type: Protein Quantitation Assay
Quantity: 275 mL
Specificity: Not Target-Specific
Sufficient For: 250 Tube Assays
Detection Method: Colorimetric
Unit Size: Each