Thermo Fisher, 23280, Pierce™ Quantitative Peroxide Assay Kit (Aqueous

Pierce™ Quantitative Peroxide Assay Kit (Aqueous The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (aqueous-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.
This aqueous-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. The kit's formulation includes sorbitol, which provides sensitivity enhancement. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.
e., control) to mitigate these effects. This kit provides for maximum sensitivity with non-lipid samples.
Features of the Quantitative Peroxide Assay Kit:
•Peroxides—detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
•Convenient—no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourselfApplications:
• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipidsIn this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With this aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.
Assay: Quantitative Peroxide Assay
Description: Pierce Quantitative Peroxide Assay Kit (Aqueous)
For Use With (Application): Solution-based Detection, Absorbance
For Use With (Equipment): Spectrophotometer, Microplate Reader
Product Line: Pierce™
Product Type: Quantitative Peroxide Assay
Quantity: 100 mL
Specificity: Not Target-Specific
Sufficient For: 100 Tube Assays or 500 Microplate Assays
Detection Method: Colorimetric
Unit Size: Each