Thermo Fisher, 26111, MA(PEG)4 Methyl-PEG-Amine Compound

Quantity: 1 g Thermo Scientific Pierce MA(PEG)4 is a methyl- and amine-terminated polyethylene glycol reagent that is useful for a variety of surface-modification and molecule-pegylation applications.
Features of MA(PEG)4:
• Fully characterized PEGylation reagent with defined PEG chain length; discrete molecular weight for consistency of performance in protein-modification applications
• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
• Allows site-specific labeling of primary amines or carboxyl groups on proteins or surfaces
• Easy-to-follow instructions increase the likelihood of a successful outcomeMA(PEG)n is the abbreviation for a set of compounds having polyethylene glycol (PEG) spacers and terminal methyl (-CH3) and amino (-NH2) groups. The unbranched, hydrophilic, discrete-length molecules have the form Methyl-PEGn-Amine, where the subscript 'n' denotes 4, 8, 12, or 24 ethylene glycol units. The terminal primary amine of each compound provides a specific target for crosslinking and other conjugation methods, making these compounds useful as PEGylation reagents.
Applications of PEGylation:
• PEGylate carboxylate or amine surfaces
• Add inert mass to proteins, immunogens, drug compounds and probes
• Improve solubility (decrease aggregation) of proteins or peptides without affecting function
• Protect proteins from proteolysisMethods of Covalent Attachment:
• Conjugate to carboxyl groups usingEDCandSulfo-NHS
• Crosslink to primary amines usingDSS,BS(PEG)5/BS(PEG)9or other NHS-ester reagent
• Crosslink to sulfhydryl groups usingSulfo-SMCCorSM(PEG)nreagent
• Attach to oxidized carbohydrate groups (aldehydes) byreductive aminationWhy PEGylate a protein or peptide?Methyl-capped PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, protect it from proteolytic digestion, increase its half life in a biological application, mask it from causing an immunogenic response, decrease its antigenicity or potential toxicity, improve its solubility, diminish the potential for aggregation, and minimize interference for bothin vitroandin vivoapplications. Polyethylene glycol, also called polyethylene oxide (PEO), has these effects because it is nontoxic, nonimmunogenic, hydrophilic, water soluble and highly flexible.
Advantages of Discrete-length Polyethylene Glycol Compounds:These reagents are specially synthesized, resulting in homogeneous compounds of defined molecular weight, characterized by discrete chain lengths, providing a greater ability to optimize and characterize surface protein modifications. Typical preparations of PEG compounds are a heterogeneous mixtures composed of a distribution of chain lengths with a specified average molecular weight or approximate number of PEG subunits.
Form: Liquid
Label Type: Polyethylene Glycol (PEG), Blocking Groups
Quantity: 1 g
Reactive Moiety: Amine, Primary Amine
Solubility: Water
Chemical Reactivity: Ketone
Product Type: Amine Compound
Spacer: 4 ethylene glycol units
Unit Size: Each