Thermo Fisher, 26182, Pierce™ Anti-HA Agarose

5 mL Thermo Scientific Pierce Anti-HA Agarose is an immunopurification and immunoprecipitation resin for the enrichment of HA-tagged proteins expressed inhuman in vitro protein expression systems, bacteria, yeast, and mammalian cells. The anti-HA antibody coupled to the resin is a high-affinity mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein.
Features of Pierce Anti-HA AgaroseSpecific—highly specific anti-HA monoclonal antibody enables high yield and high purityScalable—available in 1 and 5 mL settled resin package sizes to allow for smaller scale immunoprecipitations or larger scale purificationsVersatile—can be used in spin or gravity-based purification and enrichment methodsPierce Anti-HA Agarose consists of highly specific monoclonal anti-HA antibody that is covalently immobilized on a crosslinked 4% beaded agarose support. The product is supplied as a 50% slurry in 0.
1 M phosphate, 0.
15 M NaCl, pH 7.
2 with 0.
05% sodium azide. The anti-HA agarose has 3.
5 mg mouse anti-HA IgG1 monoclonal antibody conjugated per mL of settled agarose resin.
HA-tagged proteins are easily eluted from the resin using 0.
1 M glycine (pH 2.
0–2.
8), 3 M NaSCN, or 50 mM NaOH, depending on the downstream application of the purified protein. For gentle elution,HA Synthetic Peptideis available for use in neutralization and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.
Related productsPierce HA-Tag IP/Co-IP KitPierce HA-Tag Magnetic IP/Co-IP KitConjugate: HA-epitope tag (YPYDVPDYA)
Purification Target: Anti-HA Antibody
Quantity: 5 mL
Column Type: Agarose Resin, Affinity
Format: Bottle
Product Type: Agarose
Unit Size: Each