Product Description
Thermo Fisher, 27-1794-09, Phosphorylating Phosphoramidite, standard grade, 20-400 finish bottle Phosphorylating phosphoramidite can be utilized to achieve phosphorylation of the 5' oligo terminus (also known as Phosphate-ON or Chemical Phosphorylation Reagent (CPR)). Chemical phosphorylation is a cost-effective substitute to enzymatic methods (via T4 kinase and ATP), allowing efficient introduction of terminal phosphate groups. Oligos with a 5'-phosphate are conducive to a number of various applications utilizing the ligation of oligos to each other, such as NGS adapters, cloning, and gene construction. This product is supplied in a 30 mL amber bottle with a 20-400 Finish neck style, which is compatible with most Expedite Systems of oligonucleotide synthesizers.
Features:
• Full chemical name—2-[2-(4,4-Dimethoxytrityloxy)ethylsulfonyl]ethyl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
• Molecular formula—C34H45F3N2O7PS
• Formula weight—656.
66 g/mol
• HPLC Purity—≥95.
0%For Use With (Equipment): Expedite Synthesizers
Form: Powder
Grade: Standard
Nucleic Acid Type: None (Modifier)
Nucleoside Type: None (Modifier)
Product Type: Phosphorylating CEP
Purity: 95%
Quantity: 100 μmol
Reagent Type: Phosphoramidite
Shipping Condition: Room Temperature
Unit Size: Each
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Collaboration
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