Thermo Fisher, 29997, Pierce™ Recombinant Protein L, Biotinylated Thermo Scientific™

Pierce™ Recombinant Protein L, Biotinylated Thermo Scientific™ Use Thermo Scientific Pierce Biotinylated Recombinant Protein L to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.
Features of Recombinant Protein L:
• Contains four immunoglobulin-binding domains per protein
• Binds members of all classes of Ig (IgG, IgM, IgA, IgE and IgD) that contain kappa light chains
• Binds single chain variable fragments (scFv) and Fab fragments that contain kappa light chainsProtein L is an immunoglobulin-binding protein that was originally derived from the bacteriaPeptostreptococcus magnus, but is now produced recombinantly. Protein L has the unique ability to bind through kappa light chain interactions without interfering with an antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L will bind to all classes of Ig (IgG, IgM, IgA, IgE and IgD). Protein L will also bind single chain variable fragments (scFv) and Fab fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.
Properties of Recombinant Protein L:
• Source:E. coli
• Molecular Weight: ∼35,800 (Apparent MW by SDS-PAGE: 36,000)
• Form: salt-free powder
• A280 of 0.
1% solution: ∼0.
80
• Isoelectric point (pI): 4.
8Form: Powder
Protein Subtype: Protein L
Target: Antibodies
Conjugate: Biotin
Product Line: Pierce™
Product Type: Protein L, Biotinylated
Quantity: 0.
5 mg
Unit Size: Each