Thermo Fisher, 35603, Pierce™ Iodoacetic Acid

Pierce™ Iodoacetic Acid Thermo Scientific Pierce Iodoacetic Acid (IAA) can react with several protein functional groups but is typically used for specific S-carboxymethylation of sulfhydryls (reduced cysteines).
Features of Pierce Iodocetic Acid (IAA):
• React at slightly alkaline pH for specific S-carboxymethylation of free sulfhydryls
• React at low pH for specific carboxymethylation of methionines
• React at high pH to favor carboxymethylation of histidines and lysines
• Methylate reduced cysteine peptide fragments in protease digests for mass spectrometryIodoacetic acid reacts with sulfhydryls on cysteines, imidazolyl side chain nitrogens of histidines, the thioether of methionine and the primary amine group of lysines.
The rate of reaction and specificity is dependent on the ionization level, which can be manipulated by the pH of the reaction condition.
Description: Iodoacetic Acid
Form: Powder
Label Type: Blocking Groups
Label or Dye: Unreactive Group
Product Line: Pierce™
Quantity: 500 mg
Solubility: Water
Chemical Reactivity: Thiol
Product Type: Iodoacetic Acid
Spacer: Short (<10 Å)
Unit Size: Each