Thermo Fisher, 4333793, Proteinase K Solution

Thermo Fisher, 4333793, Proteinase K Solution Proteinase K from the fungusEngyodontium albumis a non-specific serine protease that is useful for general digestion of proteins.
Proteinase K Remains Active:
• Over a wide pH range (optimal activity between 6.
5 and 9.
5)
• Under denaturing conditions (e.
g. the presence of SDS or urea)
• In the presence of metal chelating agents (e.
g. EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)Applications:Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections forin situhybridization.
Performance and Quality Testing:Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit Definition:One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
For Use With (Application): Chromatin Biology/RNA Extraction/DNA Extraction
Product Type: Proteinase K
Purity: DNase-Free, RNase-Free
Quantity: 5 mL
Shipping Condition: Wet Ice
Form: Liquid
Unit Size: Each