Thermo Fisher, 4334973, SYBR™ Green PCR Master Mix

1 x 50 mL Notification of updated SYBR Green master mix formulation:PowerTrack SYBR Green Master Mixis our newest formulation designed to greatly simplify your work and deliver high sensitivity and specificity. It comes with an optional yellow dye that can be added to your DNA sample for pipetting QC. Yellow sample added to blue master mix yields green qPCR reaction. Visually confirm that your DNA sample was added to master mix.
PowerTrack SYBR Green Master Mixis compatible with a wide range of primer melting temperatures (55°C to 65°C) and concentrations to minimize primer optimization work.----------------------------------------------SYBR Green PCR Master MixEverything you need for SYBR Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems SYBR Green PCR Master Mix combines SYBR Green I dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
Premixed components stored at 2–8°C significantly reduce assay setup timeSYBR Green I dye detects double-stranded DNA, so specific probes are not requiredAmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performancedUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylaseProprietary buffer enhancements ensure performance and reliabilityMaximum flexibility and convenienceApplied Biosystems SYBR Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan probes are required. SYBR Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
Concentration: 2X
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Format: Tube
No. of Reactions: 2000 Reactions
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Product Line: SYBR™
Product Type: Real Time PCR SYBR Master Mix
Quantity: 1 x 50 mL
Sample Type: DNA (Genomic), cDNA
Shipping Condition: Wet Ice
Sufficient For: 2000 Reactions of 50 μL
Volume: 1 x 50 mL
Detection Method: SYBR
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
Label or Dye: SYBR Green
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each