Thermo Fisher, 4398967, TaqMan™ Rodent MicroRNA A Array v2.0

TaqMan™ Rodent MicroRNA A Array v2.
0 The Applied Biosystems™ TaqMan™ Rodent MicroRNA A Array v2.
0 contains 384 TaqMan™ MicroRNA Assays enabling accurate quantitation of 335 and 226 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan™ MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan™ MicroRNA Assay not related to human is included as a negative control. Details of the included assays is provided in the Array Map file. Purchase of the Megaplex™ RT Primers, Rodent Pool A is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool A for situations where sensitivity is of the utmost importance and/or sample is limiting.
TaqMan™ MicroRNA Arraysprovide all the advantages of TaqMan™ MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan™ MicroRNA Assays in parallel.
Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the the TaqMan™ MicroRNA Array v2.
0. When sensitivity is of the utmost importance, and/or sample is limiting Megaplex™ PreAmp Primers provide an optional preamplification step. TaqMan™ MicroRNA Arrays v2.
0 and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with Sanger miRBase v10 to be created in as little as 5 hours, providing the ideal miRNA profiling solution.
Simplify Sample Handling and Increase Sample ThroughputFollowing reverse transcription of miRNA targets using Megaplex RT Primers and optional preamplification using Megaplex PreAmp Primers, TaqMan™ Universal PCR Master Mix is simply combined with each reaction and pipetted into each of the eight sample loading ports in the TaqMan™ Array, simplifying sample handling and increasing sample throughput.
Provides Comprehensive Coverage of Sanger miRBase v10Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan™ MicroRNA Arrays v2.
0. The content is derived from miRBase v10, and provides close to full coverage of Sanger with the most up-to-date TaqMan™ MicroRNA Assays. Each array (either human or rodent) contains a selection of carefully chosen endogenous control assays, providing you with a variety of options for data normalization. Of particular note is the inclusion of a new mammalian U6 assay, repeated four times on each card, and an assay unrelated to mammalian species, ath-miR159a, providing a process control.
Ideal for miRNA ProfilingThe highly streamlined workflow—coupled with the high specificity, sensitivity, and dynamic range of TaqMan™ Assays—make this product solution ideal for miRNA profiling, especially when compared to microarrays.
For Use With (Equipment): AB 7900 HT 384-Well System
Form: Dried Down
Includes: 384-Well Arrays, Information CD
Internal Probe Modification: MGB (Minor Groove Binder) (3in), Non-Fluorescent Quencher (3in), FAM (5in)
Label or Dye: FAM
Product Line: TaqMan™
Quantity: 4 pack
Reaction Speed: Standard
Research Category: Gene Regulation, Epigenetics
Type: Rodent MicroRNA A Array
Format: 384-well Microfluidic Card
Number of Arrays: 4 arrays
Species: Mouse, Rat
Unit Size: Each